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Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli |
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| Authors: | Misun Won Soyoun Kim Seunghwan Lee Chuljung Kim Hyunsu Kim Moohyung Jun Kyung Bin Song |
| Affiliation: | (1) Cell Cycle and Signal Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea;(2) Departments of Food Science and Technology, Korea;(3) Veterinary Medicine, Chungnam National University, Taejon, 305-764, Korea |
| Abstract: | To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied. |
| Keywords: | MBP-fusion protein Salmonella entiritidis SEF 17 |
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