Immunochemical aspects of the screening of gene expression

Different immunochemical methods of gene expression screening in the heterologous system are described. Special attention is paid to the technique of in situ screening and to the methods of the product detection in lysates and cell colony replicas on the basis of immunoblot, immunodot, immunoprecipitation and immunochemical analysis on plates. The data on cell lysis, solid-phase immobilization and sensitivity of different immunochemical methods are discussed.