Effect of L-cystine on macromolecular changes during spore and parasporal crystal formation inBacillus thuringiensis var.thuringiensis |
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Authors: | S Rajalakshmi Y I Shethna |
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Institution: | (1) Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560 012 Bangalore |
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Abstract: | High concentration of L-cystine (0.25%) when present in a glucose-mineral salt medium inhibited sporulation-specific events
like protease production, calcium uptake and dipicolinic acid synthesis inBacillus thuringiensis var.thuringiensis. In addition, the enzymes of the Krebs cycle from aconitase onwards were completely inhibited by a high concentration of cystine.
At a low concentration of cystine (0.05%), none of the above mentioned macromolecular changes were affected. Lipid synthesis
monitored by 1,214 C]-acetate incorporation into lipid as well as into whole cells was completely inhibited. |
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Keywords: | Bacillus thuringiensis spore crystal macromolecular changes |
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