加拿大披碱草和圆柱披碱草BC1植株的细胞遗传学鉴定

以加拿大披碱草(2n=4x=28)和圆柱披碱草(2n=6x=42)为材料,对其BC1植株染色体数目、配对构型以及花粉育性和结实性等进行了鉴定。结果显示:BC1代85%以上细胞染色体数目为28条(2n=4x=28);BC1植株的花粉母细胞减数分裂中期Ⅰ染色体配对行为较规则,其平均染色体构型分别为0.04Ⅰ 13.98Ⅱ,且环状二价体多于棒状二价体;BC1植株的花粉可育率和自然结实率分别为84.83%和70.98%,说明BC1植株的育性已得到恢复,为其后代优良株系的选育奠定了基础。

Identification of Chromosome and Fertility of BC1 between Elymus canadensis and E.cylindricus

With Elymus canadensis L.(2n=4x=28) and Elymus cylindricus (Franch.) Honda (2n=6x=42) as tested plants,the chromosome number,chromosome pairing configuration,pollen fertility and seed set of BC1 were analyzed.The results showed that the chromosome numbers in above 85% root tip cell of BC1 plant are 28,which are tetraploid(2n=4x=28);the chromosome pairing behavior at metaphase Ⅰ in pollen mother cells of BC1 plant are regular,and the average chromosome pairing configurations are 0.04Ⅰ+13.98Ⅱ,and the frequency of ring bivalent was obviously more than rod bivalent;the pollen fertility and the seed set of BC1 plant are 84.83%and 70.98%respectively.It was proved that the fertility of BC1 plant had been restored.The above results provide a basis for selection and breeding of progenies.