胃促胰酶和肥大细胞在豚鼠过敏性休克死亡的应用

目的:建立豚鼠过敏性休克模型,研究胃促胰酶和肥大细胞在过敏性休克诊断上的应用。方法:20只清洁级豚鼠随机分为10只实验组和10只对照组,应用混合人血清构建的过敏模型,ELISA方法测定豚鼠血清Ig E含量,免疫组化染色观察胃促胰酶在喉头、气管、肺、胃、肠的表达,肥大细胞特殊染色计数肥大细胞。结果:实验组豚鼠有70%发生过敏性休克死亡,实验组豚鼠血清中Ig E的含量显著高于对照组豚鼠(P0.05),实验组豚鼠于喉头、气管、肺胃促胰酶的表达高于对照组(P0.05),实验组豚鼠于喉头、气管、肺、胃的肥大细胞总数高于对照组豚鼠(P0.05),肺组织观察到肥大细胞脱颗粒。结论:胃促胰酶和肥大细胞可以为过敏性休克死亡的法医学鉴定提供参考。

Application of Chymase and Mast Cell in Guinea Pig Death of Anaphylactic Shock

Objective:To establish guinea pig anaphylactic shock model and explore the application of chymase and mast cell in diagnosing anaphylactic shock.Methods:20 healthy guinea pigs were random classified into experiment group (n=10) and control group (n=10). The mixed human serum-induced anaphylactic shock model was established. The ELISA was used to detect the serum IgE in guinea pig. Immunohistochemistry was used to detect the expression of chymase in larynx, trachea, lung, stomach and bowel. Toluidine blue was used to count mast cells.Results:70 percent of guinea pigs died due to anaphylactic shock. The serum IgE of experiment group was significantly higher than that of control group, P<0.05, and chymase-positive mast cells were found in the larynx, trachea, lung and not found in the stomach and bowel. There was significant difference between the two groups, P<0.05. The total mast cells were found in the larynx, trachea, lung and stomach, P<0.05 compared with control group.The degradulation of mast cells was found in the lung.Conclusion:The chymase and mast cells can assist in forensic diagnosis of anaphylactic shock