Characteristics of NADH-dependent lipid peroxidation in sarcoplasmic reticulum of white shrimp,Litopenaeus vannamei,and freshwater prawn,Macrobrachium rosenbergii |
| |
Authors: | Lin Yu-Ruh Huang Sue-Lan Huang Chen-Huei |
| |
Institution: | a National Chiayi University, Department of Aquatic Biosciences, 300 University Road, Chiayi 600, Taiwan;b National Chiayi University, Department of Food Science, Chiayi, Taiwan |
| |
Abstract: | The iron-catalyzed NADH-dependent lipid peroxidation system in sarcoplasmic reticulum (SR) of cultured white shrimp, Litopenaeus vannamei and freshwater prawn, Macrobrachium rosenbergii was characterized. Production of thiobarbituric acid reactive substances was used to measure the activity of lipid peroxidation. In both species, the system preferred NADH to NADPH as the reducing agent. Lipid peroxidation activities of SR from both species increased when reaction temperatures increased from 6 to 26 °C. At 66 °C, the reaction was no longer NADH-dependent. Acidic pH amplified the lipid peroxidation activity. Sarcoplasmic reticular lipid peroxidation activity in white shrimp was always greater than in freshwater prawn. Fatty acid composition of SR lipids could be a major factor for this outcome. The proportion of n–3 highly unsaturated fatty acids, such as C20:5 and C22:6, in sarcoplasmic reticular lipids of white shrimp was twice of that in freshwater prawn. The results of this study provide important tools required for anti-oxidative nutrient study at sub-cellular level. |
| |
Keywords: | Fatty acid profile Freshwater prawn Lipid peroxidation Litopenaeus vannamei Macrobrachium rosenbergii NADH Sarcoplasmic reticulum White shrimp |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|