Polysome formation and stability in pea stem and root tissue |
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Authors: | Sylwia?Krupińska Email author" target="_blank">Stanis?aw?WeidnerEmail author Ewa?Fr?czek Ryszard?Amarowicz |
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Institution: | (1) Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn-Kortowo, Oczapowskiego St. 1A, Poland;(2) Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima St. 10, P.O. Box 55, 10-718 Olsztyn 5, Poland |
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Abstract: | Polysome stability and the formation of various polysomal populations in pea stem and root tissue were examined. Both total
ribosomal fraction and four polysome populations were isolated: FP (free polysomes), MBP (membrane-bound polysomes), CBP (cytoskeleton-bound
polysomes) and CMBP (cytoskeleton-membrane-bound polysomes). The content of above mentioned populations decreased in roots
and stems during germination. In both roots and stems a gradual decrease of FP participation in the total polysomal population
was also observed during germination. On the other hand, an obvious increase in participation of CMBP population in the total
polysomes pool was observed in later stages of germination. Increase of CMBP participation in pea root and stem tissues in
later stages of germination is probably due to intensive enzymatic protein synthesis taking place in them. These proteins
may participate in elongating growth of cells. The results of investigation on polysomes stability showed that total polysomes
isolated from pea roots appeared to be more resistant to digestion by exogenous ribonuclease (EC 3.1.27.5) than polysomes
isolated from stems. As protein-mRNA interactions are widely known and ribosomes are also very adhesive structures, numerous
non-ribosomal proteins are present in the polysome preparations. We suppose that changes in proteins bound to polysomes indicated
by us previously, significantly influence both the stability and also translatability of polysomes isolated from different
plant organs. |
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Keywords: | Pisum sativum L polysome stability cytoskeleton-membrane-bound polysomes germination pea root stem |
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