Evidence for a plasmid conferring salt-tolerance in the plant-root associated,diazotrophKlebsiella sp. NIAB-I |
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Authors: | Javed A Qureshi Kauser A Malik |
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Institution: | (1) National Institute of Biotechnology & Genetic Engineering(NIBGE), Jhang Road, P.O. Box 577, Faisalabad, Pakistan;(2) Nuclear Institute for Agriculture & Biology (NIAB), Jhang Road, P.O. Box 128, Faisalabad, Pakistan |
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Abstract: | Summary Using analytical and preparative methods, we demonstrated the presence of an indigenous plasmid (pNIAB-I) in a diazotroph,Klebsiella sp. NIAB-I isolated, from the roots of Kallar grass, growing on saline lands in Pakistan. The plasmid is approximately 50 kilobase (kb) in size. Transformation experiments indicated that non-halophilic bacteria such asE. coli K12 strain (MV10) andK. pneumoniae M5AI on acquiring this plasmid become tolerant to high salt (NaCl) and alkaline pH. |
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