The effect of biochanin A on the chlorophylls and carotenoids content in the alga <Emphasis Type="Italic">Chlorella vulgaris</Emphasis> Beijerinck |
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Authors: | Romuald Czerpak Alicja Piotrowska Paweł Dobrzyń Andrzej Tatur Monika Marczuk |
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Institution: | (1) Institute of Biology, University of Białystok, Swierkowa 20 B, 15 - 950 Białystok;(2) Institute of Ecology, Polish Academy of Sciences, Dziekanów Leśny, 05 - 092 Łomianki |
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Abstract: | The present study was undertaken to determine the influence of biochanin A, isoflavone characterised by estrogenic activity,
upon the content of chlorophylls and carotenoids in the cells of green alga Chlorella vulgaris Beijerinck (Chlorophyceae). On the 6th day of cultivation under the influence of 10−6 M biochanin A exerted the greatest biological activity and the most stimulating effect on the analysed parameters: growth
of the alga expressed by the cells number and the content of photosynthetic pigments in them. The total content of carotenoids
was stimulated on the 6th day of experiment in the range of 197 % but during the 9th day only in 179 % in comparision with the control group (100 %). At the same time content of carotenes increased to the level
of 123 – 119 % and xanthophylls to 208 – 178 %. Among the carotenes, β-carotene was characterised with the 3.7 times higher
content in regard to the content of α-carotene on the 6th day of cultivation and during the 9th day — the 5.7 times domination. The content of xanthophylls that contain two atoms of oxygene in molecule (oxygen — poor
xanthophylls) was intensively stimulated in the range of 224 %. Moreover, the oxygen — rich xanthophylls content reached the
value 179 % when compared to the control. The greatest stimulation of the content of chlorophylls and its isomers was observed
during the 3rd day of cultivation of Chlorella vulgaris when it rose up to 166 % and to 156 % on the 6th day. The content of chlorophyll b and its isomers was stimulated in 181 % on the 6th day of culture and 155 % during the 9th day of algal culture. The evidence on the stimulating effect of biochanin A as the main representative of isoflavones on
the growth and content photosynthetic pigments in eucaryotic alga C. vulgaris was demonstrated in these studies. |
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Keywords: | Chlorella vulgaris biochanin A growth chlorophylls a and b carotenes xanthophylls |
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