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Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli |
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| Authors: | Jun Wu Xuewei Liao Fangbo Yu Zhongbo Wei Liuyan Yang |
| Affiliation: | 1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People’s Republic of China 4. Department of Environmental Biology, School of the Environment, Nanjing University, 163 Xianlin Avenue, Nanjing, Jiangsu Province, 210046, People’s Republic of China 2. Center for Analysis and Testing, Nanjing Normal University, Nanjing, People’s Republic of China 5. Department of Environmental Sciences, School of Environment and Resource Sciences, Zhejiang Agriculture and Forestry University, Linan, Zhangjiang Province, 311300, People’s Republic of China |
| Abstract: | A dibutyl phthalate (DBP) transforming bacterium, strain M673, was isolated and identified as Acinetobacter sp. This strain could not grow on dialkyl phthalates, including dimethyl, diethyl, dipropyl, dibutyl, dipentyl, dihexyl, di(2-ethylhexyl), di-n-octyl, and dinonyl phthalate, but suspensions of cells could transform these compounds to phthalate via corresponding monoalkyl phthalates. During growth in Luria–Bertani medium, M673 produced the high amounts of non-DBP-induced intracellular hydrolase in the stationary phase. One DBP hydrolase gene containing an open reading frame of 1,095 bp was screened from a genomic library, and its expression product hydrolyzed various dialkyl phthalates to the corresponding monoalkyl phthalates. |
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