Osmotic adjustment of Zymomonas mobilis to concentrated glucose solutions |
| |
Authors: | Theodor Struch Burkard Neuss Stephanie Bringer-Meyer Hermann Sahm |
| |
Institution: | (1) Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, Postfach 19 13, W-5170 Jülich, Germany |
| |
Abstract: | Summary The physiological basis of the exceptionally high sugar tolerance of Zymomonas mobilis was investigated. Determinations of the internal metabolite concentrations of Z. mobilis showed that an increase in the extracellular glucose concentration was accompanied by a parallel rise in the intracellular glucose concentration, bringing about an almost complete osmotic balance between internal and external space. Studies of glucose transport confirmed that Z. mobilis has a facilitated diffusion system which enables a rapid equilibration between internal and external glucose concentrations. Studies using the non-metabolisable sugars maltose (impermeable) and xylose (permeable) revealed that these sugars were able to alter the osmotic pressure on the cytoplasmic membrane resulting in volume changes.Dedicated to Professor R. K. Finn on the occasion of his 70th birthday |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |