MMP2和MMP9在粘膜内胃癌中表达及其临床病理意义

目的:观察基质金属蛋白酶(MMP)家族成员MMP2和MMP9在粘膜内胃癌中的表达及其与淋巴结转移的相关性。方法:研究病例为病理诊断为粘膜内胃癌的档案病例,应用免疫组织化学技术检测MMP2和MMP9在粘膜内胃癌中表达的临床病理意义,特别是与淋巴结转移的相关性。结果:临床病理分析结果显示有淋巴结转移的IMGC病例肿块直径要显著大于无淋巴结转移的IMGC。有淋巴结转移IMGC中低分化腺癌发生率要显著高于无淋巴结转移组。有淋巴结转移IMGC中淋巴管侵犯发生率要显著高于无淋巴结转移组。免疫组化结果显示,MMP2在正常胃粘膜上皮和粘膜内胃癌中的阳性表达率分别是7%和43.93%,有显著性差异(P0.01),MMP9在正常胃粘膜上皮和粘膜内胃癌中的阳性表达率分别为和23%和48.48%,无显著性差异(P0.05)。MMP9在淋巴结转移组中的阳性率(87.5%)显著高于无淋巴结转移组(36%),在有淋巴管侵犯病例中的表达率(83.3%)显著高于无淋巴管侵犯的病例(30%),差异均有统计意义(P0.05);而MMP2的表达与有无淋巴结转移及淋巴管侵犯均无显著相关性(P0.05)。结论:MMP9可能作为预测粘膜内胃癌是否有淋巴结转移的标志物,但需要结合组织分化、肿块大小和淋巴管侵犯等临床病理特点综合判断。MMP2可能与粘膜内胃癌的发生有关而作为早期诊断的指标。

Clinicopathological Significancer of MMP2 and MMP9 Expression in the Intramucosal Gastric Carcinoma

Objective： To investigate the expression of MMP2 and MMP9 in the intramucosal gastric carcinoma and its correlation with the lymphatic metastasis. Methods： Samples involved in this study were diagnosed as intramucosal gastric carcinoma by clinicopathological daignosis. The MMP2 and MMP9 expression were assayed by immunohistochemical technique; the correlation of which with lymphatic metastasis were analyzed. Results： Clinicopathological feature were that lump size was larger in IMGC with lymph node metastasis than that in IMGC without lymph node metastasis. The incidence of Poorly poorly differentiated IMGC was higher in IMGC with lymph node metastasis than that in IMGC without lymph node metastasis. The incidence of Lymphatic permeationwas higher in IMGC with lymph node metastasis than that in IMGC without lymph node metastasis. Immunohistochemical results showed that positive rate of MMP2 in normal mucosal epithelial and intramucosal gastric carcinoma were 7% and 43,93% with significant difference （P〈0.01）,positive rate of MMP2 in normal mucosal epithelial and intramucosal gastric carcinoma were 23% and 48.48% without significant difference（P〉0.05）. The positive rate of MMP9 expression was higher in IMGC with lymph node metastasis（87.5%）than that in the IMGC without lymph node metastasis （36%）; the positive rate of MMP9 expression was higher in IMGC with lymphatic permeation （83.3%） than in the IMGC without lymphatic permeation （30%）. No significant correlation of MMP2 expression with the lymph node metastasis and lymphatic permeation was found （P〉0.05）. Conclusion： MMP9 expression could be used as a biomarker for the prediction of lymph node metastasis in IMGC, which should be analyzed combined with tisuue diferentiation, lump size and lymphatic permeation. MMP2 could be associated with development of intramucosal gastric carcinoma and become an index for early daignosis.