链霉菌A048产几丁质酶最佳发酵工艺研究

将链霉菌A048在完全培养基中培养至对数生长末期，离心洗涤收集菌丝体，然后接种入发酵产酶培养基中，进行二步发酵工艺牛产几丁质酶，几丁质酶活力比一步发酵工艺提高1．1倍，发酵周期共54h，比一步发酵工艺缩短66h；把菌丝体与几丁质粉共固定化，接入发酵产酶培养基中培养36h，几丁质酶活力比一步发酵工艺提高1．8倍，发酵周期缩短54h；在二步发酵工岂中另添加0．4％纤维素，几丁质酶活力可提高4倍，比一步发酵工艺提高10倍，酶活力达18．52U／mL。采用几丁质和纤维索双因子诱导二步发酵工艺可能是链霉菌A048生产几丁质酶的最佳工艺。

Study on the Optimal Fermentation Process for Production Chitinase of Streptomyces sp. A048

Streptomyces sp. A048 was cultured in a complete medium to the last stage of log phase, the hyphae were washed and collected by centrifugation. Then the hyphae were inoculated in liquid medium for chitinase production using two-step fermentation. Activity of chitinase produced by two-step fermentation was 1.1 times higher than that from one-step fermentation, and ferment cycle was for 54 hours, which was 66 hours shorter than that of one-step fermentation. The hyphae and the powder of chitin were co-immobilizated and cultured in liquid medium for 36 hours, activity of chitinase was 1.8 times higher than that from one-step fermentation, and ferment cycle was 54h shorter than that of one-step fermentation. By adding 0, 4% cellulose to two-step fermentation, actiw ity of chitinase was 18.52 U/mL that was 4 times higher than that from the control and 10 times higher than that from one-step fermentation. Two step fermentation with chitin and cellulose may be the optimal fermentation process to produce Chitinase from Streptomyces sp. A048.