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Chronic TNFα and cAMP pre-treatment of human adipocytes alter HSL, ATGL and perilipin to regulate basal and stimulated lipolysis |
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| Authors: | Véronic Bézaire Aline Mairal Rodica Anesia Corinne Lefort Dominique Langin |
| Institution: | a Laboratoire de Recherches sur les Obésités, Inserm U858, Toulouse, France b Université de Toulouse, UPS, Institut de Médecine Moléculaire de Rangueil, IFR150, Toulouse, France c CHU de Toulouse, Laboratoire de Biochimie, Institut Fédératif de Biologie de Purpan, Toulouse, France |
| Abstract: | We examined the effects of chronic TNFα and dibutyryl-cAMP (Db-cAMP) pre-treatment on the lipolytic machinery of human hMADS adipocytes. TNFα decreased adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) protein content and triglycerides (TG)-hydrolase activity but increased basal lipolysis due to a marked reduction in perilipin (PLIN) protein content. Conversely, Db-cAMP increased ATGL and HSL protein content but prevented PLIN phosphorylation, the net result being accentuated basal lipolysis. In forskolin-stimulated conditions, TNFα and Db-cAMP pre-treatment decreased stimulated TG-hydrolase activity and impaired PLIN phosphorylation. Together, this resulted in a severely attenuated response to forskolin-stimulated lipolysis. |
| Keywords: | ACS acyl-CoA synthase ATGL adipose triglyceride lipase Bay 4-isopropyl-3-methyl-2-[1-(3-(S)-methyl-piperidin-1-yl)-methanoyl]-2H-isoxazol-5-one DG diglycerides Db-cAMP dibutyryl-cyclic AMP FK forskolin hMADS cells human adipose tissue derived-multipotent stem cells HSL hormone-sensitive lipase JNK c-jun NH2 terminal kinase LD lipid droplet MAPK mitogen-activated protein kinase NF-κB nuclear factor κB NPRA natriuretic peptide receptor A OA oleic acid PAT Perilipin adipophilin TIP-47 PDE3B phosphodiesterase 3B PKA protein kinase A PLIN perilipin TG triglycerides |
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