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Drosophila big brain does not act as a water channel, but mediates cell adhesion |
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| Authors: | Kimiko Tatsumi Shoji Tsuji Hideki Miwa Mutsuo Nuriya Kazunari Kaneko Masato Yasui |
| Affiliation: | a Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan b Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan c Department of Pediatrics, Kansai Medical University, 2-3-1 Shin-machi, Hirakata-shi, Osaka 573-1191, Japan |
| Abstract: | The neurogenic gene Drosophilabig brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis. |
| Keywords: | Big brain Aquaporin-4 (AQP4) Cell adhesion Drosophila |
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