A nucleolar protein RRS1 contributes to chromosome congression |
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| Authors: | Arni E. Gambe Hideaki Takata Akiko Baba Kiichi Fukui |
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| Affiliation: | a Laboratory of Dynamic Cell Biology, Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871 Osaka, Japan
b National Institute of Genetics, 1111 Yata, Mishima, 411-8540 Shizuoka, Japan |
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| Abstract: | We report here the functional analysis of human Regulator of Ribosome Synthesis 1 (RRS1) protein during mitosis. We demonstrate that RRS1 localizes in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. RNA interference experiments revealed that RRS1-depleted cells show abnormalities in chromosome alignment and spindle organization, which result in mitotic delay. RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression. |
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| Keywords: | RNAi, RNA interference siRNA, small interfering RNA GFP, green fluorescent protein H3S10ph, phospho-histone H3 serine 10 |
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