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Birth of common marmoset (Callithrix jacchus) offspring derived from in vitro-matured oocytes in chemically defined medium
Authors:I Tomioka  T Takahashi  A Shimada  K Yoshioka  E Sasaki
Affiliation:a Department of Applied Developmental Biology, Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan
b Center for Integrated Medical Research, Keio University School of Medicine, Tokyo, Japan
c Pathology and Pathophysiology Research Division, National Institute of Animal Health, Ibaraki, Japan
d Department of Biochemistry and Integrative Medical Biology, Keio University School of Medicine, Tokyo, Japan
Abstract:Optimization of oocyte culture conditions is a crucial aspect of reproductive biology and technology. In the present study, maturation of germinal vesicle-stage marmoset oocytes were evaluated in the following media: Waymouth medium, Waymouth medium containing porcine follicular fluid (pFF) (Waymouth-pFF medium), and porcine oocyte medium (POM). Oocytes cultured in Waymouth-pFF medium had higher maturation rates to the metaphase II stage than those cultured in Waymouth medium (36.1% vs. 24.8%, respectively, P < 0.05), indicating the suitability of this medium for culturing marmoset oocytes. Hence, maturation of marmoset oocytes cultured in POM was subsequently evaluated. The rate of maturation to the metaphase I stage was significantly higher and degradation rates were significantly lower in oocytes cultured in POM than those cultured in Waymouth medium. In addition, three offspring were successfully obtained after transfer of embryos matured in chemically defined medium. Therefore, we concluded that POM was suitable for marmoset oocyte culture. Furthermore, this was apparently the first report of marmoset offspring derived from oocytes cultured in chemically defined medium.
Keywords:Assisted reproductive technology   Chemically defined medium   Common marmoset   In vitro culture   In vitro fertilization   In vitro maturation
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