Efficient detection of PrPSc (263K) in human plasma. |
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| Authors: | Ryo Fujita Takeru Urayama Akimasa Ohmizu Mikihiro Yunoki Shoutaro Tsuji Kazuyoshi Ikuta |
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| Institution: | Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. |
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| Abstract: | The potential contamination of human blood or plasma with prions, such as variant Creuftzfelt-Jacob disease (vCJD), is becoming a serious problem. In this study, we established a Western blot-based detection method for PrP(Sc) (263K) spiked in plasma. Although plasma contains a large amount of protein, specific detection of a small amount of 263K in plasma could be specifically detected only after ultra-centrifugation followed by heat-denaturation of plasma proteins included in the resulting precipitate, before the digestion with proteinase K. |
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