At least two regions of the oncoprotein Tre2 are involved in its lack of GAP activity |
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Authors: | Bizimungu Christelle Vandenbol Micheline |
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Institution: | Animal and Microbial Biology Unit, Gembloux Agricultural University, B-5030 Gembloux, Belgium. bizimungu.c@fsagx.ac.be |
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Abstract: | The product of the human Tre2 oncogene is structurally related to the Ypt/Rab GTPase-activating proteins (Ypt/Rab GAPs). Particularly, the oncoprotein shares with the yeast proteins Msb3p and Msb4p, and with the human protein RN-tre the highly conserved TBC domain, forming the catalytically active domain of Ypt/Rab GAPs. Yet, the Tre2 oncogene seems to encode a nonfunctional Rab GAP. As regions flanking the TBC domain may be crucial for catalytic activity, regions located N- and C-terminally with respect to this domain were explored. For this, chimeric proteins created by sequence exchanges between the Tre2 oncoprotein and RN-tre were tested for their ability to replace functionally the Msb3p and Msb4p proteins in double-mutant yeast cells. These complementation experiments revealed, in addition to the TBC domain, a second Tre2 region involved in the oncoprotein's lack of GAP activity: a 93-aa region flanking the TBC domain on the C-terminal side. |
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Keywords: | GTPase-activating protein Tre2/TRE17/USP6 oncogene RN-tre MSB3/GYP3 MSB4/GYP4 Yeast functional complementation |
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