Pherophorins: a family of extracellular matrix glycoproteins fromVolvox structurally related to the sex-inducing pheromone

Pherophorins are extracellular matrix (ECM) glycoproteins from Volvox that share homology with the sex-inducing pheromone. A novel pherophorin (pherophorin III) was characterized both with respect to expression pattern and proteolytic processing in vivo. Furthermore, it was shown that the pherophorins represent a protein family of ECM glycoproteins exhibiting a modular composition: their N-terminally located domain is a homolog of a domain found in the ECM glycoprotein SSG 185. Together with SSG 185, pherophorin I is a main component of the cellular zone within the ECM. The Volvox genome contains a tandem arrangement of genes encoding pherophorin II-related polypeptides. Inhibition of proteolytic processing of pherophorin II and III in vivo appears to result in the suppression of sexual induction.Abbreviations ECM extracellular matrix - PCR polymerase chain reaction - RACE rapid amplification of cDNA endsWe wish to thank Dr. R. Deutzmann (Universität Regensburg, Germany) for sequencing peptides. This work was supported by the Deutsche Forschungsgemeinschaft (SFB43).