Enhanced colchicine production in root cultures of Gloriosa superba by direct and indirect precursors of the biosynthetic pathway |
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| Authors: | Biswajit Ghosh Swapna Mukherjee Timir B Jha Sumita Jha |
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| Institution: | (1) Centre of Advanced Study, Department of Botany, University of Calcutta, Calcutta;(2) Present address: RKMVC College, Rahara, 24-Parganas (North), W.B., India |
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| Abstract: | Excised root cultures of Gloriosa superba reached 7.5 g dry wt l–1 and accumulated 240±40  g colchicine g–1 cell dry wt after 4 weeks growth. While all precursors (except trans-cinnamic acid) enhanced colchicine content of root cultures without adversely affecting root growth, treatment with p-coumaric acid + tyramine (each at 20 mg l–1) increased colchicine content to 1.9 mg g–1 cell dry wt. |
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| Keywords: | colchicine Gloriosa superba root culture |
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