荧光标记金针菇原生质体融合

以金针菇（Flmmulina velutipes）为材料，经异硫氨酸荧光素（FIC）标记的金针菇单核W19菌株的原生质体与未经标记的单核Y7菌株的原生质体在聚乙二醇（PEG）的诱导下进行融合，利用显微操纵仪直接挑取一个带有荧光而另一个不具荧光的原生质体对，培养后，根据融合形成的汉核菌丝具有“锁状连合”这一形态学特征选择、鉴定融合菌株，酯酶同工酶电泳分析结果表明，融合菌株具双亲互补酶带。经测定，多数融合菌株菌丝生长速度和生物量较亲株高，且出菇正常。

STUDES ON FUSON OF FLUORESCEIN-LABELED PROTOPLASTS FROM FLAMMULINA VELUTIPES

This paper studied the fusion of fluorescein-labeled protoplastS of two kinds ofFlommulina velutipes. The Fluorescein iso-thiocyanate (FITC)-labeled protoplasts of strain W19and the non-labeled protoplasts of strain Y7 could be induced to fuse in the presence ofpolyethylene glycol (PEG). A pair of sticked protoplasts which only one labeled could beselected direchy using whcromahipulator. We screened the fusant from the regenerated coloniesby the clamp connechon of dikaryophyte. Anlysising the esteraes isozyme, we found theisozyme bands of the fusants were complemental with those of their parentS. The growth rateof the mycelia and the biomass 0f many fusantS were superior to those of their parents.