Purification and Properties of Alcohol Oxidase from Candida methanosorbosa M-2003 |
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Authors: | Shin-ichiro Suye |
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Institution: | (1) Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 3-9-1, Bunkyo, Fukui 910, Japan , JP |
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Abstract: | Alcohol oxidase from Candida methanosorbosa was
purified about sixfold with a yield of 37.6% from the culture broth of
Candida methanosorbosa M-2003. The enzyme preparation was homogeneous
on slab gel electrophoresis. The purified enzyme had an optimal pH from 6.0
to 9.0 and was stable in the range 6.0–8.5. Its optimal temperature of
reaction was 50°C, and it was stable below 50°C. In the presence of
NaN3, the enzyme retained its initial activity at 30°C for 35
days, indicating stability for a long term, so far. The isoelectric point was
pH 4.3. Its molecular weight was 620,000 by gel filtration chromatography and
80,000 by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. These
results indicate that the enzyme consists of 8 subunits.
Received: 1 October 1996 / Accepted: 12 December 1996 |
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