Improving the Effectiveness of Artificial MicroRNA (amiR)-Mediated Resistance against Turnip Mosaic Virus by Combining Two amiRs or by Targeting Highly Conserved Viral Genomic Regions |
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| Authors: | Guillaume Lafforgue Fernando Martínez Qi-Wen Niu Nam-Hai Chua José-Antonio Daròs Santiago F. Elena |
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| Affiliation: | Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, València, Spaina;Laboratory of Plant Biology, Rockefeller University, New York, New York, USAb;The Santa Fe Institute, Santa Fe, New Mexico, USAc |
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| Abstract: | A drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs (amiRs) is the ease with which viruses generate escape mutations. Here, we show two alternative strategies for improving the effectiveness of resistance in plants. First, we expressed two amiRs complementary to independent targets in the viral genome, and second, we designed amiRs complementary to highly conserved RNA motifs in the viral genome. |
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