Strand-specific DNA degradation in a mutant of Escherichia coli |
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Authors: | George E Veomett and Peter L Kuempel |
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Institution: | (1) Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, USA |
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Abstract: | Summary A dna B mutant of Escherichia coli which is thermosensitive for DNA synthesis at 42° C degrades DNA at the restrictive temperature. The degradation specifically affects newly synthesized DNA, begins at the replication forks and proceeds toward the replication origin, and is limited to 10–15% of one chromosome. The parameters of DNA degradation, as well as DNA-DNA annealing experiments on newly synthesized DNA which is resistant to degradation, indicate a specific strand of newly synthesized DNA is degraded. |
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