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Improvement of isoflavone aglycones production using β-glucosidase secretory produced in recombinant Aspergillus oryzae |
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| Authors: | Ken Horii Takashi Adachi Tetsuya Matsuda Tsutomu Tanaka Hiroshi Sahara Seiji Shibasaki Chiaki Ogino Yoji Hata Mitsuyoshi Ueda Akihiko Kondo |
| Abstract: | β-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than β-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides. |
| Keywords: | β -Glucosidase Isoflavone Aglycone Aspergillus oryzae Superoxide dismutase (sodM) promoter |
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