| 人SR-AI转基因小鼠遗传与繁育的研究 |
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| 引用本文： | 王宗保,万腊香,吴端生,刘鑫,杨永宗.人SR-AI转基因小鼠遗传与繁育的研究[J].中国实验动物学报,2001,9(3):129-133. |
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| 作者姓名： | 王宗保 万腊香 吴端生 刘鑫 杨永宗 |
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| 作者单位： | 1. 南华大学实验动物学部
2. 南华大学分子生物学研究中心 |
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| 基金项目： | 本课题为国家自然科学基金资助项目(编号:39900061) |
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| 摘 要： | 目的探讨被转入的人SR-AI基因的整合与复制情况及其对小鼠繁育的影响.方法采用类似系祖建系的方法,对人SR-AI转基因小鼠的2、3534、3560、3638、3639五个系列进行了繁育.并用PCR和Southermblot的方法,检测五个系列小鼠尾组织的DNA样品.结果五个系列小鼠共产仔431只,PCR检出阳性小鼠178只,阳性率为41.2%.在3639系的F1、F2、F3代及纯合子转基因鼠中PCR阳性率分别为47.8%、71.3%、75%和100%.结论人SR-AI基因在子代鼠中能稳定遗传,且对生殖和发育无明显影响.
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| 关 键 词： | 转基因小鼠 人清道夫受体AI基因 繁育 基因整合 |
| 文章编号： | 1005-4847(2001)03-0129-05 |
| 修稿时间： | 2000年11月10 |
Study on Heredity and Breeding of Human Scavenger Receptor-AI Transgenic Mice |
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| WANG Zongbao,WAN Laxiang,WU Duansheng,LIU Xin,YANG Yongzong.Study on Heredity and Breeding of Human Scavenger Receptor-AI Transgenic Mice[J].Acta Laboratorium Animalis Scientia Sinica,2001,9(3):129-133. |
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| Authors: | WANG Zongbao WAN Laxiang WU Duansheng LIU Xin YANG Yongzong |
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| Abstract: | Objective To investigate human SR AI transgenic integration and reproduction and its effect on mice breeding. Methods Human SR AI transgenic mice models 2, 3534,3560,3638,3639 have been bred by means of creating the system similar to the system ancestry. PCR and Southern blot techniques were used to test the DNA samples of tail tissues in the 5 lines of mice. Results In 431 baby mice of the 5 lines, 178 were tested by PCR to be positive (41.2%) while the positive rates of generations F 1, F 2, F 3 in line 3639 and pure zygote transgnic mice were 47.8%,71.3%,75% and 100% respectively. Conclusion Human SR AI gene can steadily heritable in the mice sub generation and doesn't influence obviously their reproduction and growth. |
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| Keywords: | Transgenic mice Human SR AI gene Breeding Gene integration |
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