| 有机溶剂对缢蛏碱性磷酸酯酶分子构象和催化活力的影响 |
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| 引用本文： | 刘红兵,颜思旭,程玉华.有机溶剂对缢蛏碱性磷酸酯酶分子构象和催化活力的影响[J].中国生物化学与分子生物学报,1993,9(4):500-506. |
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| 作者姓名： | 刘红兵 颜思旭 程玉华 |
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| 作者单位： | 厦门大学生物系，吉林大学酶工程实验室，吉林大学酶工程实验室 厦门 361005 福建省亚热带植物研究所 361006 |
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| 摘 要： | 本文初步研究了三种有机溶剂甲醇、二甲基甲酰胺、二氧六环对缢蛏碱性磷酸酯酶(ALP)分子构象和催化活力的影响。发现在有机溶剂作用下,ALP的荧光发射光谱、紫外差光谱、园二色光谱都发生了显著的变化,而酶经甲醇、二甲基甲酰胺预处理后酶活力提高,经二氧六环预处理后活力下降,但当测活体系中有三种有机溶剂存在时,酶的活力却被大大抑制。
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| 关 键 词： | 碱性磷酸酶 有机溶剂 分子构象 催化活力 |
| 收稿时间： | 1993-08-20 |
The Influence of Organic Solvent on the Molecule Conformation and Catalytic Activity of the Alkaline Phosphatase from Sinonovacula Constricta |
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| Liu,Hong-bing Yan,Si-xu Cheng,Yu-hua.The Influence of Organic Solvent on the Molecule Conformation and Catalytic Activity of the Alkaline Phosphatase from Sinonovacula Constricta[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(4):500-506. |
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| Authors: | Liu Hong-bing Yan Si-xu Cheng Yu-hua |
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| Institution: | (Department of Biology, Xiamen University; Lab of Enzymic Engineering, Jilin University |
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| Abstract: | The influence of three kinds of organic solvents(methanol, N,N'-dimethyl-formamide and dioxane) ou the molecule conformation and catalytic activity of the alkaline phosphatase(ALP) from Sinonovacula Constricta was studied. The fluorescence,circular dichroism spectra and ultraviolet differential spectra of ALP changed distinctty in the presence of the mentioned organic solvent media. The activity of ALP increasd markedly after pretreatment with methanol and N,N'-dimethyl. On the contrary, they decreased after the pretreamment with dioxane. But the activity of ALP decreased markedly when the three organic solvent present in the assay system. |
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| Keywords: | Alkaline phosphatase Organic solvent Molecule conformation Catalytic activity |
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