Serotonin induced actin polymerization and association with cytoskeletal elements in cultured bovine aortic endothelium |
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| Authors: | J S Alexander H B Hechtman D Shepro |
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| Institution: | Department of Medical Cell Biology, University of Uppsala, Sweden. |
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| Abstract: | Serotonin, (5-hydroxytryptamine, 5-HT) binds to cultured endothelial cell stress fibers as identified by fluorescence microscopy and in vitro induces actin polymerization as measured by DNase 1 inhibition and differential centrifugation; the structural change in actin in the presence of 5-HT resembles actin paracrystals as visualized by negative staining under electron microscopy. These observations indicate that 5-HT, which is taken up by endothelium by a non-mediated diffusion, may interact directly with actin to affect cytoskeletal changes. |
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