Highly regenerative tissue cultures from axillary tiller buds of sexually mature oat plants (Avena sativa L.)

Summary Competent tissue cultures were initiated from axillary tiller buds and immature leaves of two cultivars ofAvena sativa L. and cultured on agar nutrient medium containing 2 mg/l of 2,4-dichlorophenoxyacetic acid and 1 mg/l benzyladenine. Using a technique of selective excision and subculturing of the shoot-forming tissues and rejecting the root-froming tissues, we regenerated numerous plants either on hormone-free medium or by allowing the subculture with hormone to age under usual culture-room light conditions. This research was supported in part through a grant to A. W. G. from BARD (Binational Agricultural Research and Development Foundation). N. S. S. is grateful to the Ministry of Education and Culture, Government of India, New Delhi, for the award of a National Scholarship for study abroad 1980–81.