33-kDa C-terminal heparin binding fragment of fibronectin promotes attachment and spreading of hepatocytes |
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Authors: | N Anil Kumar P R Sudhakaran |
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Institution: | (1) Department of Biochemistry, University of Kerala, 695 581 Kariavattom, Trivandrum, India |
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Abstract: | The possibility of interaction of hepatocytes with the heparin binding domain of Fibronectin was examined. Rat hepatocytes
adhered to coverslips coated with the 33-kDa heparin binding fragment of the C-terminal region of plasma fibronectin. When
different concentrations of the heparin binding fragment were used to coat coverslips and used as substratum, cell attachment
showed saturation kinetics. Half the maximum attachment was observed at 30–40 min after seeding of cells. The cells became
flat after 2–3 h indicating that they spread on the heparin binding domain as they do on intact fibronectin. Among the different
glycosaminoglycans tested, maximum inhibition of attachment was observed for heparin. However it was not possible to completely
inhibit attachment even at high concentrations. These results indicate that hepatocytes interact with fibronectin not only
through the Arg-Gly-Asp-containing cell binding fragment, but also through the heparin binding domain of fibronectin and,
further, that there exist heparin-dependent and heparin-independent mechanisms of interaction of cells with the 33-kDa heparin
binding fragment of fibronectin |
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Keywords: | Heparin binding domain of fibronectin hepatocytes cell attachment and spreading |
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