Direct somatic embryogenesis and plant regeneration from single cell suspension cultures of Elymus giganteus Vahl |
| |
Authors: | Li Wang Xiaoguang Wang Baiqu Huang |
| |
Institution: | (1) Institute of Genetics and Cytology, Northeast Normal University, 130024 Changchun, The People's Republic of China |
| |
Abstract: | Summary A yellowish, nodular callus was induced from mature embryos of Elymus giganteus Vahl on MS medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l kinetin, from which a cell suspension culture was initiated in liquid MS medium supplemented with 0.5 mg/l 2,4-D, 1.0 mg/l kinetin and 0.2 mg/1 naphthaleneacetic acid (NAA). By filtering through a series of sieves with decreasing mesh sizes and collecting the resultant filtrate, a suspension culture composed mainly of single embryogenic cells was established. In a medium containing 0.3 mg/l 2,4-D, 1.0 mg/l 6-benzylaminopurine (6-BAP) and 500 mg/l casein hydrolysate (CH), the single cells underwent direct somatic embryogenesis resulting in the formation of proembryos. These proembryos developed into mature embryos when placed in a double-layer liquid overlay culture. Intact plants were developed from somatic embryos when they were transferred onto solidified MS medium without added growth regulators. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|