The chimeric cytokine Hyper-IL-6 enhances the efficiency of lentiviral gene transfer in hepatocytes both in vitro and in vivo |
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Authors: | Virgínia Picanço-Castro Aparecida Maria Fontes Stefan Heinz Torsten Tonn Dimas Tadeu Covas |
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Institution: | 1.Hemocentro de Ribeir?o Preto,Centro de Terapia Celular – FMRP/USP,Ribeir?o Preto,Brazil;2.Institute for Transfusion Medicine and Immunohaematology, Red Cross Blood Donor Service Baden-Wuerttemberg-Hesse,Johann Wolfgang Goethe University Clinics,Frankfurt/Main,Germany |
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Abstract: | Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes
remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6
in the lentiviral transduction. The lentiviral vector was used to transduce HepG2 cells and mice liver cells, previously treated
with H-IL6. The highest transduction level was observed in HepG2 cells treated with 30 ng/mL H-IL6 and in the mice that received
4 μg H-IL6. Our results suggest that H-IL6 is an inducer of lentiviral gene transfer into the liver cells without any toxicity. |
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Keywords: | Gene therapy Hepatocytes H-IL6 Lentiviral vectors Transduction efficiency |
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