Differential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP. |
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| Authors: | Dong-Jae Kim Jong-Hwan Park Seung-Hyeok Seok Sun-A Cho Min-Won Baek Hui-Young Lee Ki-Hwa Yang Dong Deuk Jang Beom-Seok Han Jae-Hak Park |
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| Institution: | Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Korea. |
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| Abstract: | Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion in the PCR product from M. arthritidis. This method might be useful to differentiate infection of M. pulmonis from that of M. arthritidis. |
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