Enzymes and coenzymes of the carbon monoxide dehydrogenase pathway for autotrophic CO2 fixation in Archaeoglobus lithotrophicus and the lack of carbon monoxide dehydrogenase in the heterotrophic A. profundus |
| |
Authors: | Julia Vornolt Jasper Kunow Karl O Stetter Rudolf K Thauer |
| |
Institution: | (1) Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität und Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany;(2) Lehrstuhl für Mikrobiologie, Universität Regensburg, Universitätsstrasse 33, D-93053 Regensburg, Germany |
| |
Abstract: | Archaeoglobus lithotrophicus is a hyperthermophilic Archaeon that grows on H2 and sulfate as energy sources and CO2 as sole carbon source. The autotrophic sulfate reducer was shown to contain all the enzyme activities and coenzymes of the reductive carbon monoxide dehydrogenase pathway for autotrophic CO2 fixation as operative in methanogenic Archaea. With the exception of carbon monoxide dehydrogenase these enzymes and coenzymes were also found in A. profundus. This organism grows lithotrophically on H2 and sulfate, but differs from A. lithotrophicus in that it cannot grow autotrophically: A. profundus requires acetate and CO2 for biosynthesis. The absence of carbon monoxide dehydrogenase in A. profundus is substantiated by the observation that this organism, in contrast to A. lithotrophicus, is not mini-methanogenic and contains only relatively low concentrations of corrinoids.Abbreviations
F
420
coenzyme F420
-
MFR
methanofuran
-
CHO-MFR
formylmethanofuran
-
H
4MPT
5,6,7,8-tetrahydromethanopterin
-
CHO–H
4MPT N5
formyl-H4MPT
- CHH4MPT+N5
methenyl-H4MPT
-
CH
2=H4MPT N5, N10
methylene-H4MPT
-
CH
3–H4MPT N5
methyl-H4MPT
-
H
4F
tetrahydrofolate
-
I U
1 mol/min
-
t
d
doubling time |
| |
Keywords: | Methanofuran Tetrahydromethanopterin Coenzyme F420 Corrinoids Cytochromes Autotrophic CO2 fixation Dissimilatory sulfate reduction Archaeoglobus species Methanogenic Archaea |
本文献已被 SpringerLink 等数据库收录! |
|