N—acetylserotonin对肝缺血再灌注小鼠氧化应激损伤的保护作用

目的探讨NAS对肝缺血再灌注所诱导的脂质过氧化损伤产生的保护作用。方法采用夹闭肝蒂法30min、再灌注6h制作肝缺血再灌注模型，冰冻切片，HE染色，光学显微镜下观察肝细胞形态结构的变化；比色法检测损伤后血清中谷丙转氨酶（ALT）水平及肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH—Px）的含量。结果夹闭肝蒂30min、再灌注6h后，肝小叶结构紊乱、肝血窦淤血，其间有白细胞浸润、肝细胞出现变性、坏死；血清中ALT水平升高，肝组织中s0D和GSH—Px的含量降低，MDA升高；NAS可减少缺血再灌注后血清ALT的释放，使肝组织中SOD和GSHPx的含量升高，MDA的含量降低；NAS＋Luz可逆转NAS的这一作用。结论NAS对肝缺血再灌注小鼠的氧化应激损伤具有保护作用。

Antioxidant effects of N-acetylserotonin on mice during hepatic ischemia reperfusion injury

Objective To investigate the antioxidant effects of NAS on mice during hepatic ischemia reperfusion injury. Methods The model of hepatic ischemia reperfusion injury was made by clamping the hepatic artery, portal vein, and common bile duct for 30min with microvascular bulldog clamps and open- ning them for 6h. The morphologic changes were observed by light microscopy. The serum concentration of ALT was measured by methods recommended by the manufacturer. The terminal productions of lipid peroxidation malondialdehyde （MDA） and anti-oxidase series superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） were assayed. Results In the IR group, the pathological examination showed de- rangement of hepatic lobules, congestion of hepatic sinusoids, leukocytic infiltration,degeneration and necrosis of hepatocytes. The serous ALT was obviously higher than that of the sham group, together with the increase of MDA,and decrease of SOD and GSH-Px. Those changes in the NAS group were obviously milder than those in the IR group, and Luz reversed them. Conclusion NAS has antioxidant effects on mice during hepatic ischemia reperfusion injury.