不同悬浮介质对激光衍射法测得的变形指数—渗透压曲线的影响

本文用PBS和PVP两种介质作为激光衍射仪的悬浮介质,系统地观测了悬浮介质物化性能不同对红细胞变形指数(DI)—渗透压曲线的影响,发现:1.在不同悬浮介质中的变形指数—渗透压曲线有显著差别,在高渗区这种差别更明显.2.同一红细胞试样,在相同高渗压下,在不同的悬浮介质中进行交叉实验表明、PBS悬浮介质能使得红细胞变形性得到恢复,而PVP悬浮介质却使得红细胞变形性显著降低.3.在高渗情况下,首次观察到红细胞变形性随时间变化,一开始DI增大,约3小时后趋于稳定,无论PBS还是PVP其趋势皆相似.

THE INFLUENCE OF PBS SUSPENDING MEDIUM ON EKTACYTOMETER OSMOSCAN CURVE

Ektacytometer osmoscan ourve desoribing erythrocyte deformability index (DI) in'relation to Inoresing osmolality of the suspending buffer were studied and osmoscan curve for PBS suspending medium has been obtained for the first time. The authers found that different influences of high viscosity suspending medium (PBS) on Ektacytometer osmoscan curve exist. However, erythrocyte de-formabillty in both high osmotio suspending media increases with the time.The results read as follows:1 .Ektaoytometer osmosoan curve for PBS suspending mediun is different from that for dextran, no minimum DI exists in low osmotic range of Ektacytometer osmoscan curve, namely, the DI doesn't increase with decrease of osmotic pressure.2.DI in high osmotio pressure range of Ektaoytometer osmoscan curve for PBS suspending medium decreases slowly i.e. Ektacytometer curve in high osmotio range becomes broader than that for dextran suspending medium.3.Ektacytometric measurements interchanging for PBS suspending medium and dextran suspending medium testified that crythrooyte deformabiliy in PBS suspending medium is better than that in dextran suspending medium in the high osmotic pressure.