Identification and subgrouping of Frankia strains using sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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Authors: | M Gardes M Lalonde |
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Institution: | Centre de Recherche en Biologie Forestière, Facultéde Foresterie el de Géodésie, Univ. Laval, Ste-Foy, Québec. Canada G1K 7P4. |
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Abstract: | Polypeptide patterns of soluble proteins from 35 Frankia strains from different plants of various geographical origins, belonging to Alnus and Elaeagnus host-specificity groups were determined by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The polypeptide pattern was qualitatively the same for each strain whatever the number of subcultures or the age. Two gel electrophoresis groups A and E were observed which matched with the Alnus and Elaeagnus host-specificity groups, but with some exceptions. The polypeptide patterns of the 35 Frankia strains tested were separated into 13 gel electrophoresis subgroups. Five Frankia strains were inoculated separately or in 3 mixed combinations of 2 strains on Alnus glutinosa (L.) Gaertn. plants. The polypeptide patterns of the re-isolates obtained from 5-month-old nodules were identical to the corresponding strains used initially in the inoculum. Dual infection was observed on single plantlets. |
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Keywords: | Protein patterns SDS-PAGE taxonomy |
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