Unreduced diploid nuclei in Cupressus dupreziana A. Camus pollen |
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| Authors: | C Pichot M El Maâtaoui |
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| Institution: | (1) INRA, Unité de Recherches Forestières Méditerranéennes, Equipe de Génétique Av. Vivaldi, 84000 Avignon, France e-mail: pichot@avignon.inra.fr Fax: +33(0)4 90 13 59 59, FR;(2) Université d’Avignon, Faculté des Sciences, Département de Biologie, 33 rue L. Pasteur, 84000 Avignon, France, FR |
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| Abstract: | Size and DNA content of pollen of Cupressus dupreziana A. Camus, a highly endangered Mediterranean conifer, were assessed by cytomorphological observations and flow cytometric analyses and
then compared to C. sempervirens L. pollen. Mature C. dupreziana pollen was composed of two uninucleated types of pollen grains differing in size. Around 35% of the grains exhibited a size
similar to C. sempervirens pollen, while 65% exhibited a larger diameter. However only one peak of fluorescence was detected by flow cytometry. DNA
content of C. dupreziana pollen was twice the DNA content of C. sempervirens pollen. Comparison of DNA contents of mature and germinating pollen revealed that mature pollen of both species were arrested
in the G2 phase. Comparison with the DNA content of somatic tissue (2C) provided evidence for the production of unreduced
pollen in C. dupreziana. This unexpected feature in gymnosperms is discussed in terms of reproductive strategy of this species.
Received: 11 December 1999 / Accepted: 22 December 1999 |
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| Keywords: | 2n pollen Cupressus dupreziana Gymnosperms Flow cytometry Reproduction |
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