皂荚种质资源SCoT遗传多样性分析及指纹图谱的构建

采用SCoT标记分析了18个皂荚种质的遗传多样性,并采用UPGMA法对18个皂荚种质进行了聚类分析。在此基础上,通过筛选出的多态性条带构建了18个皂荚种质的SCoT指纹图谱。扩增结果表明:从51个SCoT引物中筛选了15个引物进行PCR扩增,共扩增出226条带,其中多态性条带216条,多态性比率为96.61%。各引物多态性信息含量(PIC)、观测等位基因数(Na)、有效等位基因数(Ne)、Nei’s基因多样性指数(H)和Shannon’s信息指数(I)的平均值分别为0.875 9、1.964 9、1.440 1、0.272 6、0.426 1。18个皂荚种质的遗传相似系数在0.491 4~0.938 1之间,表明供试材料之间具有较丰富的遗传多样性。聚类分析结果表明:在遗传相似系数为0.60处可将18个皂荚种质分为3组,其中野皂荚单独为一组,山皂荚和皂荚-T聚为一组,其它皂荚材料聚为一组。利用3个引物扩增的8个多态性位点构建了18份皂荚种质资源的DNA指纹图谱,可以将其区分并精准鉴定。该研究结果为皂荚种质的鉴定和新品种选育提供了一定的理论依据。

Genetic diversity and fingerprints of Gleditsia sinensis germplasm resource based on SCoT

Genetic diversity of eighteen Gleditsia sinensis germplasms was analyzed based on SCoT markers, the cluster analysis on eighteen G. sinensis germplasms was carried out by UPGMA method, and the SCoT fingerprints of eighteen G. sinensis germplasms were constructed by the polymorphic bands. The results of amplification showed that, 226 SCoT bands were obtained from fifteen primers selected from 51 SCoT primers, including 216 polymorphic bands, with a polymorphism rate of 96.61%. The average of the polymorphism information content(PIC), observed number of alleles(Na), effective number of alleles(Ne), Nei''s gene diversity(H)and Shannon''s information index(I)were 0.875 9, 1.964 9, 1.440 1, 0.272 6 and 0.426 1, respectively. The genetic similarity coefficients of these samples were between 0.491 4 and 0.938 1. Therefore, it indicates that there are rich genetic diversities among these materials. The results of cluster analysis showed that, eighteen G. sinensis germplasms could be divided into three groups when the genetic similarity coefficient was 0.60. G. heterophylla formed the first group, G. melanacantha and Zaojia-T formed the second group, and the others formed the third group. DNA fingerprints of eighteen samples were constructed from eight polymorphic loci amplified by three primers, and these materials could be distinguished and identified accurately. All these results provide the important information for the identification and breeding for new cultivars of G. sinensis.