A chimaeric hygromycin resistance gene as a selectable marker in plant cells |
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| Authors: | Peter J. M. van den Elzen Jeffrey Townsend Kathleen Y. Lee John R. Bedbrook |
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| Affiliation: | (1) Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, 94608 Oakland, CA, U.S.A.;(2) Present address: Dept. of Genetics, Free University, De Boelelaan 1087, 1081 Amsterdam, The Netherlands |
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| Abstract: | Summary We show here that plant cells are sensitive to the antibiotic hygromycin-B4. We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments. |
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| Keywords: | Ti plasmid Hygromycin transformation |
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