Microarray image analysis: background estimation using quantile and morphological filters |
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Authors: | Anders Bengtsson and Henrik Bengtsson |
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Institution: | (1) Mathematical Statistics, Centre for Mathematical Sciences, Lund University, Box 118, SE-221 00 Lund, Sweden |
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Abstract: | Background In a microarray experiment the difference in expression between genes on the same slide is up to 103 fold or more. At low expression, even a small error in the estimate will have great influence on the final test and reference
ratios. In addition to the true spot intensity the scanned signal consists of different kinds of noise referred to as background.
In order to assess the true spot intensity background must be subtracted. The standard approach to estimate background intensities
is to assume they are equal to the intensity levels between spots. In the literature, morphological opening is suggested to
be one of the best methods for estimating background this way. |
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Keywords: | |
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