UCP2 protects hypothalamic cells from TNF-alpha-induced damage |
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| Authors: | Degasperi Giovanna R Romanatto Talita Denis Raphael G P Araújo Eliana P Moraes Juliana C Inada Natália M Vercesi Aníbal E Velloso Lício A |
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| Institution: | Department of Internal Medicine, University of Campinas, DCM, FCM, UNICAMP, 13084-970 Campinas, SP, Brazil. |
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| Abstract: | Uncoupling protein 2 (UCP2) is highly expressed in the hypothalamus; however, little is known about the functions it exerts in this part of the brain. Here, we hypothesized that UCP2 protects hypothalamic cells from oxidative and pro-apoptotic damage generated by inflammatory stimuli. Intracerebroventricular injection of tumor necrosis factor alpha (TNF-alpha)-induced an increase of UCP2 expression in the hypothalamus, which was accompanied by increased expression of markers of oxidative stress and pro-apoptotic proteins. The inhibition of UCP2 expression by an antisense oligonucleotide enhanced the damaging effects of TNF-alpha. Conversely, increasing the hypothalamic expression of UCP2 by cold exposure reversed most of the effects of the cytokine. Thus, UCP2 acts as a protective factor against cellular damage induced by an inflammatory stimulus in the hypothalamus. |
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| Keywords: | CAT catalase ICV intracerebroventricular IP intraperitoneal ROS reactive oxygen species SOD superoxide dismutase TNF-α tumor necrosis factor alpha TUNEL terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling UCP2 uncoupling protein 2 |
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