IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans |
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| Authors: | Marie Lopez Christo P Christov María Dolores Sequedo Mareike Herzog Anna E Herlihy Maxime Bodak Roxani Gatsi Howard A Baylis |
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| Affiliation: | 1. Department of Zoology, University of Cambridge, Cambridge, UK;2. Research Group in Molecular, Cellular and Genomic Biomedicine, Health Research Institute‐La Fe, Valencia, Spain;3. Centre for Biomedical Network Research on Rare Diseases (CIBERER), Valencia, Spain |
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| Abstract: | RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5‐trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up‐regulating IP3 signalling decreases sensitivity. Tissue‐specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment. |
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| Keywords: |
C. elegans
calcium signalling enhanced RNAi inositol 1,4,5‐trisphosphate RNA interference |
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