Continuous production of large amounts of monoclonal immunoglobulins in hollow fibers using protein-free medium |
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Authors: | F Dhainaut N Bihoreau J L Meterreau J Lirochon R Vincentelli G Mignot |
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Institution: | (1) Direction de la Recherche et du Développement, Innovation Therapeutique, CNTS, 3 Av. des Tropiques, BP 100, F-91943 Les Ulis, France |
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Abstract: | The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.Abbreviations CHES
2-(N-Cyclohexylamino)ethane-sulfonic acid
- ECS
Extracapillary Space
- FSCE
Free Solution Capillary Electrophoresis
- HPSEC
High Performance Size Exclusion Chromatography
- ICS
Intracapillary Space
- MAb
Monoclonal Antibody
- PFM
Protein-Free Medium
- SFM
Serum-Free Medium
- SSM
Serum-Supplemented Medium |
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Keywords: | continuous culture free solution capillary electrophoresis hollow fibers monoclonal antibody characterization protein-free medium |
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