Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells |
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Authors: | Nadja Zeltner Fabien G Lafaille Faranak Fattahi Lorenz Studer |
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Institution: | 1.Developmental Biology, Center for Stem Cell Biology, Sloan-Kettering Institute for Cancer Research;2.St. Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University |
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Abstract: | Human pluripotent stem cells (hPSCs) have great potential for studying human embryonic development, for modeling human diseases in the dish and as a source of transplantable cells for regenerative applications after disease or accidents. Neural crest (NC) cells are the precursors for a large variety of adult somatic cells, such as cells from the peripheral nervous system and glia, melanocytes and mesenchymal cells. They are a valuable source of cells to study aspects of human embryonic development, including cell fate specification and migration. Further differentiation of NC progenitor cells into terminally differentiated cell types offers the possibility to model human diseases in vitro, investigate disease mechanisms and generate cells for regenerative medicine. This article presents the adaptation of a currently available in vitro differentiation protocol for the derivation of NC cells from hPSCs. This new protocol requires 18 days of differentiation, is feeder-free, easily scalable and highly reproducible among human embryonic stem cell (hESC) lines as well as human induced pluripotent stem cell (hiPSC) lines. Both old and new protocols yield NC cells of equal identity. |
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Keywords: | Neuroscience Issue 87 Embryonic Stem Cells (ESCs) Pluripotent Stem Cells Induced Pluripotent Stem Cells (iPSCs) Neural Crest Peripheral Nervous System (PNS) pluripotent stem cells neural crest cells in vitro differentiation disease modeling differentiation protocol human embryonic stem cells human pluripotent stem cells |
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