| Institution: | 1. Center for Promoting Next-Generation Highly Advanced Medicine, Tottori University Hospital, 36-1 Nishicho, Yonago, Tottori, 683-8504, Japan;2. Division of Pathological Biochemistry, Department of Biomedical Sciences, Faculty of Medicine, Tottori University, 86 Nishicho, Yonago, Tottori 683-8503, Japan;3. Animal Research Facility, Advanced Medicine & Translational Research Center, Organization for Research Initiative and Promotion, Tottori University, 86 Nishicho, Yonago, Tottori 683-8503, Japan;4. Department of Plastic and Reconstructive Surgery, Faculty of Medicine, Tottori University, 36-1 Nishicho, Yonago, Tottori 683-8504, Japan;5. Division of Regenerative Medicine and Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Tottori University Graduate School of Medical Science, 86 Nishicho, Yonago, Tottori, 683-8503, Japan |
| Abstract: | In the present study, we have established a novel transgenic mouse and transgenic rats with dual reporters of EGFP and ELuc. In these transgenic (Tg) rodents, both GFP fluorescent and luciferase luminescent signals were ubiquitously detected in the heart, liver, kidney and testis, while only the GFP signal was detected in the brain. This expression system is based on a P2A linked EGFP/ELuc protein allowing both signals to be generated simultaneously. Microscopy experiments, FCM, and luciferase assays showed strong expression in freshly isolated ADSCs from Tg rodents upon transplantation of Tg rat-derived ADSCs into wild-type-mice. The ELuc transgene signal was observed and traced in vivo, and EGFP positive cells could be recovered from ELuc positive tissues in engraftment sites of wild-type mice for multiple analysis. These dual reporter Tg rodents are a useful reconstituted model system of regenerative medicine and are a valuable tool to study stem cells. |
