Surface Attachment Induced Production of Antimicrobial Compounds by Marine Epiphytic Bacteria Using Modified Roller Bottle Cultivation |
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Authors: | Liming Yan Kenneth G Boyd J Grant Burgess |
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Institution: | (1) Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, U.K., GB |
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Abstract: | A modified roller bottle culture method elicited the production of antimicrobial compounds from 2 epibiotic marine bacterial
strains, EI-34-6 and II-111-5, isolated from the surface of the marine alga Palmaria palmata. These isolates, tentatively
identified as Bacillus species, were grown as a biofilm on the surface of nutrient glycerol ferric agar (NGFA) and marine
Columbia glycerol agar (MCGA) on the inside of a rolling bottle. The biofilm was shown to be stable, and the cells were difficult
to remove from the agar surface. The culture supernatant exhibited a different antibiotic spectrum when the strains were grown
using the agar roller bottle method compared with shake flask cultures or nonagar roller bottle cultures. These results suggest
that biofilm formation is an important factor in the production of antimicrobial compounds by these 2 strains, and roller
bottle cultivation also allowed production of these compounds to be increased. The methodology used here has the potential
to allow increased production of useful secondary metabolites such as antibiotics from marine epibiotic bacteria. |
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