Induction and preliminary characterization of a novel halophage SNJ1 from lysogenic Natrinema sp. F5 |
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Authors: | Mei Yunjun Chen Jin Sun Dongchang Chen Dong Yang Yang Shen Ping Chen Xiangdong |
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Affiliation: | State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China. |
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Abstract: | Halophage SNJ1 was induced with mitomycin C from Natrinema sp. strain F5. The phage produces plaques on Natrinema sp. strain J7 only. The phage has a head of about 67 nm in diameter and a tail of 570 nm in length and belongs morphologically to the family Siphoviridae. The phage is strongly salt dependent; NaCl concentration affects the integrity of SNJ1, phage adsorption, and plaque formation. The optimal NaCl concentration for phage adsorption and plaque formation is 30% and 25%, respectively. |
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