硫酸镁对脑源性肺损伤综合征的影响

目的探讨不同剂量的硫酸镁(MgSO4)对大鼠脑源性肺损伤后神经源性肺水肿、血浆炎性因子TNF-α及肺组织病理形态学变化的影响。方法将30只SD雄性大鼠按随机数字法分为假手术组(A组)、模型组(B组)及硫酸镁50mg/kg干预组(C组)、硫酸镁100mg/kg干预组(D组)、硫酸镁200mg/kg干预组(E组),每组6只。建立大鼠颅脑损伤模型后,硫酸镁干预组即刻按50mg/kg 25%MgSO4腹腔注射,C组注射1次、D组注射2次、E组注射4次,每8h注射1次。A组及B组的大鼠注射相同剂量生理盐水作对照,C组大鼠注射1次及D组大鼠注射2次MgSO4后给予注射相同剂量的生理盐水作对照,注射方法及间隔时间同E组。伤后48h测定大鼠肺组织含水量、血浆TNF-α浓度,肺组织常规HE染色,光镜观察肺组织病理形态学变化。结果大鼠颅脑创伤后肺组织含水量均高于假手术组,以C组最明显(P0.05),差异有统计学意义。B、C、D、E组大鼠之间肺组织含水量比较差异无统计学意义。B、C、D、E组大鼠TNF-α浓度均明显高于假手术组(P0.05),D组血浆TNF-α浓度明显低于B组(P0.05),E组血浆TNF-α浓度明显低于B组(P0.01),其他各组间差异无统计学意义。假手术组肺组织形态正常,肺血管无扩张,无炎症细胞浸润;B、C、D、E组与假手术组比较均可见终末支气管腔内充满炎症细胞,周围肺组织的肺泡腔内可见炎细胞浸润,肺血管扩张、充血。B、C、D、E组在炎症细胞浸润及肺毛细血管扩张方面无明显差异。结论脑外伤可导致脑源性肺损伤综合征,可导致神经源性肺水肿;硫酸镁可降低大鼠脑损伤后血浆TNF-α浓度,对肺水肿无明显影响。

Magnesium sulfate on brain source sex lung injury syndrome

Objective To explore the different doses of magnesium sulfate on source sex rats neurogenic pulmona- ry edema after lung injury,plasma TNF-α inflammatory factors and the influence of the lung tissue pathology mor- phology. Methods 30 male SD rats by digital method were randomly divided into control group （group A） ,mod- el group （group B） and magnesium sulfate intervention group, magnesium sulfate intervention group is divided into 50 mg/kg （group C） ,100 mg/kg （group D） ,and 200 mg/kg group （group E） ,six in each group. Craniocerebral in- jury model was established, the rat made after the success of the mould, magnesium sulfate intervention group im- mediately by intraperitoneal injection of 50 mg/kg 25% MgSO4,group C injection once,twice group D and group E injection four times,getting a every 8 hours. Group A and group B the same dose of saline injection and injected one group C and group D injection after 2 times also injected the same dose of normal saline as control,group E with method. Determination of 48 hours after injury rat lung tissue water content, the plasma concentration of TNF-α, conventional HE staining, lung tissue were lung tissue pathology morphology. Results After craniocerebral trauma were lung tissue water content were higher than control group, the most obvious in group C （P〈0.05）, the difference was statistically significant. Group B,group C, D, E group there was no statistically significant difference compare between lung water content. B,C,D, E group of TNF-α concentrations were significantly higher than the control group （P〈0.05） ,plasma concentration of TNF-a D group was obviously lower than that of group B （P〈0.05） ,plasma concentration of TNF-α E group was obviously lower than that of group B （P〈0.01） ,there was no statistically significant difference between the other groups. Control form normal lung tissue, pulmonary vascular expansion, no inflammatory cell infiltration;B,C, D, E group compared with control group is visible terminal bron- chial lumen filled with inflammatory cells, lung tissue around the alveolar cavities are visible inflammatory cells in- vasion, pulmonary vasodilation and congestion. Group B, group C,D, E group between the inflammatory cells invasion and lung capillary expansion did not see obvious difference. Conclusion Traumatic brain injuries can lead to cerebral source sex lung injury syndrome, which can lead to neurogenic pulmonary edema, magnesium sulfate can reduce the damage of rats after the plasma concentration of TNF-α,had no obvious effect on pulmonary edema.